翻訳と辞書 Words near each other ・ Restricted representation ・ Restricted root system ・ Restricted Service Licence ・ Restricted shell ・ Restricted stations of Line 1, Beijing Subway ・ Restricted stock ・ Restricted sumset ・ Restricted use pesticide ・ Restricted-access barrier system ・ Restriction ・ Restriction (Archive album) ・ Restriction (mathematics) ・ Restriction digest ・ Restriction endonuclease BsobI/AvaI ・ Restriction enzyme ・ Restriction fragment ・ Restriction fragment length polymorphism ・ Restriction fragment mass polymorphism ・ Restriction landmark genomic scanning ・ Restriction map ・ Restriction modification system ・ Restriction of Hazardous Substances Directive ・ Restriction point ・ Restriction site ・ Restriction site associated DNA markers ・ Restriction-modification system ・ Restrictions (album) ・ Restrictions on cell phone use while driving in the United States ・ Restrictions on geographic data in China ・ Restrictions on political parties Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Restriction fragment ： ウィキペディア英語版 Restriction fragment A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site. Most restriction sites are palindromic, (the sequence of nucleotides is the same on both strands when read in the 5' to 3' direction), and are four to eight nucleotides long. Many cuts are made by one restriction enzyme because of the chance repetition of these sequences in a long DNA molecule, yielding a set of restriction fragments. A particular DNA molecule will always yield the same set of restriction fragments when exposed to the same restriction enzyme. Restriction fragments can be analyzed using techniques such as gel electrophoresis or used in recombinant DNA technology. In recombinant DNA technology specific restriction endonucleases are used that will isolate a particular gene and cleave the sugar phosphate backbones at different points (retaining symmetry), so that the double stranded restriction fragments have single stranded ends. These short extensions, called ''sticky ends'' can form hydrogen bonded base pairs with complementary sticky ends on any other DNA cut with the same enzyme (such as a bacterial plasmid). In agarose gel electrophoresis, the restriction fragments yield a band pattern characteristic of the original DNA molecule and restriction enzyme used, for example the relatively small DNA molecules of viruses and plasmids can be identified simply by their restriction fragment patterns. If the nucleotide differences of two different alleles occur within the restriction site of a particular restriction enzyme, digestion of segments of DNA from individuals with different alleles for that particular gene with that enzyme would produce different fragments and that will each yield different band patterns in gel electrophoresis. ==References== * 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Restriction fragment」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.